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ARTICLE
Year : 2003  |  Volume : 22  |  Issue : 4  |  Page : 134-139

In-vitro culture studies for callus and root generation of Boerhaavia diffusa Linn


1 B.R. Nahata College of Pharmacy, Mandsaur-458 001, M.P., India
2 Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur-492 010. C.G., India

Correspondence Address:
Vandana Jain
B.R. Nahata College of Pharmacy, Mandsaur-458 001, M.P.
India
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Source of Support: None, Conflict of Interest: None


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Leaf and stem explants excised from young plant of B.diffusa  were cultured on Murashige and Skoog (MS) medium containing agar (0.8%), sucrose (2.5%) and varied concentration of Indole butyric acid (IBA), Napthyl acetic acid (NAA), 2,4 - dichloro phenoxy acetic acid (2,4-D) and Picrolam. Leaf explants has given better response for both rooting and callus formation. IBA in a concentration of 5μM has shown maximum regeneration (69.7%) with induction period of 7 days. The developed roots were similar to that of naturally grown roots with little anatomical changes. For callus formation 20μM 2, 4-D has given maximum amount and percentage response 979.5% with an induction period of 8 days. Picrolam (10μM) has shown 36.6% response and the average weight of callus was less as compared with 2,4-D. The callus obtained was friable and opaque in nature.


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