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ARTICLE
Year : 2006  |  Volume : 25  |  Issue : 3  |  Page : 76-83

Refractometry as a tool in diabetic studies


1 Department of Biotechnology, Acharaya Nagarjuna University, Guntur, Andhra Pradesh, India
2 Department of Physics and Electronics, T.J.P.S College, PG Courses, Guntur, Andhra Pradesh, India

Correspondence Address:
S Kavitha
Department of Biotechnology, Acharaya Nagarjuna University, Guntur, Andhra Pradesh
India
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Source of Support: None, Conflict of Interest: None


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The refractive index as well as molar refraction, is the true index of purity of substance and plays a vital role in solution chemistry. A small addition of a foreign substance either in solid state of liquid form is going to effect the refractive index. As such the variation of refractive indices in pure glucose solution as a function of concentration is studied in detail and this principle is extended to the study of the refractive indices of urine solution of diabetic patients. The refractive indices are measured by spectrometry and abbe refractometry. A detailed study of variation of refractive indices of urine samples containing different sugar concentrations, of patients of different age groups revealed that the increase in refractive index follows a linear scale and can be explained by the equation, n=no  [l+0.00251og (a s)1/4 ] [l+0.031og0.011C]. These study provided an opportunity to project refractometry as an effective tool in diagnosing the diabetic level of a patient by making use of a simple calibration curve of increment in refractive index 'Δ n , as a function of level of the disease.


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