Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Users Online: 487 | Home Print this page Email this page Small font size Default font size Increase font size
ORIGINAL ARTICLE
Year : 2012  |  Volume : 31  |  Issue : 3  |  Page : 101-106

Anthelmintic and in vitro antioxidant evaluation of fractions of methanol extract of Leea asiatica leaves


1 Department of Pharmacology, CES College of Pharmacy, Chinnatekur, Kurnool, India
2 Department of Pharmaceutical Science, Assam University, Silchar, Assam, India
3 Director, Oil Technological Research Institute, JNTU Anantapur, Anantapur, Andhra Pradesh, India
4 Department of Pharmaceutical Chemistry, CES College of Pharmacy, Chinnatekur, Kurnool, India

Correspondence Address:
Saikat Sen
Department of Pharmacology, Creative Educational Society's College of Pharmacy, Chinnatekur, Kurnool, Andhra Pradesh
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0257-7941.103184

Rights and Permissions

Leea asiatica , a folk medicinal plant of India, is used in the treatment of worm infection and other oxidative stress-related disorders, traditionally. In the present study, the in vitro anthelmintic and in vitro antioxidant activity of different fractions of the methanol extract from the Leea asiatica leaves were evaluated. The fraction displayed significant anthelmintic activity against Indian adult earthworms (Pheretima posthuma). The ethyl acetate fraction showed a better paralysis activity (13.99 ± 0.59), while the methanol fraction showed a better death time (63.76 ± 0.73 minutes), when compared with other fractions, at a dose of 50 mg/ml concentration. The anthelmintic activity of methanol and the ethyl acetate fraction were almost similar and comparable to the standard drug, piperazine citrate. The petroleum ether fraction did not produce a potent anthelmintic effect compared to the standard. The in vitro antioxidant activity was evaluated by using the diphenyl-picrylhydrazyl (DPPH) radical scavenging assay, nitric oxide radical scavenging assay, lipid peroxidation assay, and the ferric thiocyanate method. The ethyl acetate fraction showed better antioxidant activity in all tested methods. The IC 50 value of the ethyl acetate fraction in the DPPH radical, nitric oxide radical scavenging assay, and lipid peroxidation assay were 9.5, 13.0, and 57.0 mg/ml, respectively. The fractions significantly (P < 0.05) inhibited the peroxidation of linoleic acid. The results confirmed the folk use of Leea asiatica in warm infection and the plant could be viewed as a potential source of natural anthelmintic and antioxidant compound.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2244    
    Printed82    
    Emailed1    
    PDF Downloaded236    
    Comments [Add]    
    Cited by others 3    

Recommend this journal