Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Users Online: 1078 | Home Print this page Email this page Small font size Default font size Increase font size
POSTER PRESENTATION
Year : 2012  |  Volume : 32  |  Issue : 5  |  Page : 122

PA01.72. Evaluation of DB14201 on prevention of development of streptozotocin induced diabetes mellitus in wistar rats


1 Medanta - The Medicity, Gurgaon, Haryana, India
2 Dabur Research Foundation, Sahibabad, Uttar Pradesh, India
3 Innoveda Biological Solution, New Delhi, India

Correspondence Address:
Sumit Kumari
Medanta - The Medicity, Gurgaon, Haryana
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and PermissionsRights and Permissions

Purpose: Study is part of an ongoing reverse pharmacology approach in new drug development for diabetes. It was designed to determine the potential of DB14201 to prevent the development of Streptozotocin induced Diabetes Mellitus in Wistar rats. Method: 35 female Wistar rats were randomized and allocated to different groups on the basis of body weight. G1 served as non diabetic control (Negative Control) and G2 served as Diabetic control (Vehicle Control) and received 10 ml/kg of Milli Q water. G3 and G4 were treated with 500mg/kg and 250mg/kg of DB14201 respectively. All the groups were treated orally for 21 days using disposable syringes tipped with an oral gavage needle. At the end of dosing period, hyperglycemia was induced in G2,G3 and G4 rats by intra peritoneal injection of streptozotocin (STZ) 55 mg/kg in citrate buffer (pH 4.5). Fasting blood glucose level was estimated before induction of diabetes and on 2nd & 7th days post induction. All animals were observed daily for mortality and clinical signs of toxicity throughout the experimental period. Body weight of each animal was recorded daily throughout the experimental period. On the 8th day post STZ injection, animals were killed by cervical dislocation. Their pancreatic tissues were quickly removed. Tissues were washed in normal saline and visible clots were removed to minimize blood contamination. Part of the pancreatic tissue was sent for histological evaluation. With second part of tissue, homogenates was prepared and stored at 70 A°C until the determination of biochemical parameters and enzyme activity. Result: Findings of this study strongly demonstrate that DB14201 treatment have prominent role in the prevention of STZ induced diabetes mellitus. Conclusion: Result clearly indicates the prophylactic as well as therapeutic potential of DB14201 in this experimental model. DB14201 at the dose of 500mg/kg was found to be more promising.


[PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed637    
    Printed13    
    Emailed0    
    PDF Downloaded42    
    Comments [Add]    

Recommend this journal