Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Users Online: 1022 | Home Print this page Email this page Small font size Default font size Increase font size
BRIEF REPORT
Year : 2013  |  Volume : 32  |  Issue : 3  |  Page : 173-177

Identification of bacterial endophytes associated with traditional medicinal plant Tridax procumbens Linn.


Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong, Kedah, Malaysia

Correspondence Address:
Subhash J Bhore
Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong Semeling Road, 08100 Bedong, Kedah
Malaysia
Login to access the Email id

Source of Support: Authors are grateful to the Ministry of Agriculture and Agro-Based Industry (MoA), Malaysia for fi nancial support (Research Grant Code Number: 05-02-16-SF1001), Conflict of Interest: None


DOI: 10.4103/0257-7941.123002

Rights and Permissions

Background: In traditional medicine, Tridax procumbens Linn. is used in the treatment of injuries and wounds. The bacterial endophytes (BEs) of medicinal plants could produce medicinally important metabolites found in their hosts; and hence, the involvement of BEs in conferring wound healing properties to T. Procumbens cannot be ruled out. But, we do not know which types of BEs are associated with T. procumbens. Objective: The objective of this study was to investigate the fast growing and cultivable BEs associated with T. procumbens. Materials and Methods: Leaves and stems of healthy T. procumbens plants were collected and cultivable BEs were isolated from surface-sterilized leaf and stem tissue samples using Luria-Bertani (LB) agar (medium) at standard conditions. A polymerase chain reaction was employed to amplify 16S rRNA coding gene fragments from the isolates. Cultivable endophytic bacterial isolates (EBIs) were identified using 16S rRNA gene nucleotide sequence similarity based method of bacterial identification. Results: Altogether, 50 culturable EBIs were isolated. 16S rRNA gene nucleotide sequences analysis using the Basic Local Alignment Search Tool (BLAST) revealed identities of the EBIs. Analysis reveals that cultivable Bacillus spp., Cronobacter sakazakii, Enterobacter spp., Lysinibacillus sphaericus, Pantoea spp., Pseudomonas spp. and Terribacillus saccharophilus are associated with T. procumbens. Conclusion: Based on the results, we conclude that 24 different types of culturable BEs are associated with traditionally used medicinal plant, T. procumbens, and require further study.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2874    
    Printed62    
    Emailed0    
    PDF Downloaded188    
    Comments [Add]    
    Cited by others 11    

Recommend this journal