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ORIGINAL ARTICLE
Year : 2013  |  Volume : 32  |  Issue : 4  |  Page : 227-233

Phytochemical analysis of ethanolic extract of Dichrostachys Cinerea W and Arn leaves by a thin layer chromatography, high performance thin layer chromatography and column chromatography


1 Department of Pharmaceutical Technology, Anna University, Bharathidasan Institute of Technology, Tiruchirappalli, India
2 Department of Pharmacognosy, Madurai Medical College, Madurai, India

Correspondence Address:
M Vijayalakshmi
Department of Pharmaceutical Technology, Anna University, Bharathidasan Institute of Technology, Tiruchirappalli - 620 024, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0257-7941.131978

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Background: The leaves of Dichrostachys cinerea are used as laxative, diuretic, painkiller. It is also used in the treatment of gonorrhoea, boils, oedema, gout, veneral diseases and nasopharyngeal affections, etc. Materials and Methods: The Phytochemical investigation of ethanolic extract of D. cinerea leaves were performed by standard chemical tests, thin layer chromatography (TLC) by using various solvent systems, and by high performance liquid chromatography (HPTLC). Two compounds were isolated by column chromatography and one of the compounds was identified by various spectral studies. Result : Preliminary phytochemical screening of ethanolic extract of D. cinerea leaves showed the presence of Carbohydrates, proteins, Glycosides, Saponins, Tannins, Aminoacids and Terpenoids. The TLC and HPTLC fingerprint of ethanolic extract were studied and various fractions were isolated by column chromatography and one of the fraction contain β-amyrin glucoside which was confirmed by Infra Red[IR] Spectroscopy, 1 H-Nuclear Magnetic Resonance (NMR), C- 13 NMR and Mass spectroscopic (MS) studies.


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