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ORIGINAL ARTICLE
Year : 2014  |  Volume : 34  |  Issue : 1  |  Page : 33-38

In vitro evaluation of anti-herpes simplex-1 activity of three standardized medicinal plants from Lamiaceae


1 Pharmaceutics Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
2 Herbal and Traditional Medicines Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
3 Department of Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
4 International Centers for Science and Technology and Environmental Sciences (HITEC), Kerman, Iran

Correspondence Address:
Fariba Sharififar
Herbal and Traditional Medicines Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman
Iran
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Source of Support: This work resulted from grant number 89/212. The authors would like to thank Vice Chancellor of Kerman University of Medical Sciences for supporting this project. This research was taken from Pharm.D thesis by two students (Mahnaz Heidarbeigi and Firoozeh Mehni)., Conflict of Interest: None


DOI: 10.4103/0257-7941.150777

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Background: Rosmarinic acid (RA) is a phenolic acid with antioxidant and anti-viral effects. We have studied anti-herpes simplex virus type 1 (HSV-1) effect of three medicinal plants from Lamiaceae family which have been standardized on the basis of RA content. Materials and Methods: Methanolic extract of Teucrium polium, Ziziphora clinopoides, and Salvia rhytidea was prepared by maceration method and RA content of the plants was determined using a spectrophotometric method. Maximum nontoxic concentration (MNTC) of the extracts was determined using neutral red method. Serial dilutions of extracts up to MNTC were examined on Vero cells for anti-HSV-1 effect by plaque assay in comparison to acyclovir as a positive control. Results: Among the tested extracts, T. polium contained the highest percentage of RA (1.8%w/w) and exhibited the least toxicity (MNTC = 1000 μg/ml). The greatest anti-HSV-1 was shown by T. polium and Z. clinopoides extracts which exhibited both time and concentration-dependent plaque inhibition. Conclusion: Considering the low toxicity and significant anti-viral effect of T. polium extract, this plant would prove valuable as an active anti-viral drug.


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