|Year : 2015 | Volume
| Issue : 4 | Page : 188-197
Śodhana: An Ayurvedic process for detoxification and modification of therapeutic activities of poisonous medicinal plants
Santosh Kumar Maurya1, Ankit Seth1, Damiki Laloo1, Narendra Kumar Singh1, Dev Nath Singh Gautam1, Anil Kumar Singh2
1 Department of Ayurveda Pharmacy, Ayurvedic Pharmacy Laboratory, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur, Uttar Pradesh, India
2 Department of Dravyaguna, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
|Date of Web Publication||15-Jul-2015|
Dr. Santosh Kumar Maurya
Ayurvedic Pharmacy Laboratory, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha Mirzapur - 231001, Uttar Pradesh
Source of Support: None, Conflict of Interest: None
Ayurveda involves the use of drugs obtained from plants, animals, and mineral origin. All the three sources of drugs can be divided under poisonous and nonpoisonous category. There are various crude drugs, which generally possess unwanted impurities and toxic substances, which can lead to harmful health problems. Many authors have reported that not all medicinal plants are safe to use since they can bear many toxic and harmful phytoconstituents in them. Śodhana (detoxification/purification) is the process, which involves the conversion of any poisonous drug into beneficial, nonpoisonous/nontoxic ones. Vatsanābha (Aconitum species), Semecarpus anacardium, Strychnos nux-vomica, Acorus calamus, Abrus precatorius etc., are some of the interesting examples of toxic plants, which are still used in the Indian system of medicine. Aconite, bhilawanols, strychnine, β-asarone, abrin are some of the toxic components present in these plants and are relatively toxic in nature. Śodhana process involves the purification as well as reduction in the levels of toxic principles which sometimes results in an enhanced therapeutic efficacy. The present review is designed to extensively discuss and understand the scientific basis of the alternative use of toxic plants as a medicine after their purification process.
Keywords: Ayurveda, detoxification, Śodhana, toxicity
|How to cite this article:|
Maurya SK, Seth A, Laloo D, Singh NK, Gautam DN, Singh AK. Śodhana: An Ayurvedic process for detoxification and modification of therapeutic activities of poisonous medicinal plants. Ancient Sci Life 2015;34:188-97
|How to cite this URL:|
Maurya SK, Seth A, Laloo D, Singh NK, Gautam DN, Singh AK. Śodhana: An Ayurvedic process for detoxification and modification of therapeutic activities of poisonous medicinal plants. Ancient Sci Life [serial online] 2015 [cited 2020 Aug 9];34:188-97. Available from: http://www.ancientscienceoflife.org/text.asp?2015/34/4/188/160862
| Introduction|| |
Plants are the prime source of medicine in Ayurveda. Several compounds have been isolated from medicinal plants and introduced for the service of mankind; however most of these medicines have been withdrawn due to their toxicity or side-effects. ,, Traditionally, plants having various classes of phytochemicals are still in use either in their crude form or after proper processing. Though most of the plant drugs are safe, yet few are toxic for human health. These poisonous/toxic plants are categorized as viṣa (poison) and upaviṣa (toxic but not lethal for human health) in Ayurvedic texts  [Table 1] and also listed in the schedule-E of Drugs and Cosmetics Act 1940  [Table 2]. Hence, to promote and introduce their use for medicine, such plant drugs must be detoxified or purified before their use.  The detoxification or purification process of any toxic material used for medicinal purposes is termed as "Śodhana". In Ayurveda, Śodhana is in practice since the times of Caraka Saṃhitā, but its use expanded with the development of Rasaśāstra since 8 th century CE. Śodhana process is specially designed for the drugs from mineral origin; however, it is recommended for all kinds of drugs to remove their doṣās (impurities or toxic content). It is cited in the treatises of Ayurveda that by the used of proper method of processing, viṣa can be converted into amṛta (nectar) and on other hand on adoption of inappropriate methods, nontoxic materials become a toxic.  The concept of Śodhana in Ayurveda not only covers the process of purification/detoxifcation of physical as well as chemical impurities but also covers the minimization of side effects and improving the potency/therapeutic efficacy of the purified drugs. 
|Table 2: Ayurvedic poisonous plant listed in the schedule E of drugs and cosmetics Act 1940 and rule 1945 |
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Active constituents of many plant drugs may exert severe toxic effect at high concentrations. ,, The purification processes are basically intended to reduce the toxicity level to a body sustainable limit and to reduce the toxic constituents to some extent or by potentiating their chemical transformation to nontoxic or relatively less toxic substances by enhancing their biological efficacy. Ayurvedic classics have emphasized various methods of Śodhana to overcome the undesired effects from various poisonous and nonpoisons drugs, ,, involving different media specific to substances such as Godugdha (milk of Bos indicus), Gomūtra (urine of Bos indicus), Triphalā (combination of three fruits, Terminalia chebula, Terminalia bellarica and Emblica officinalis) and lemon juice etc. , [Table 3]. A number of toxicological and pharmacological studies have been investigated on the active phytochemicals of many poisonous plants after their Śodhana. The objective of the present study is to review the state of knowledge about the Śodhana process of many poisonous plants. The present review also describes up to date information regarding the different process of detoxification (Śodhana) in Ayurvedic system of medicines.
| Various Toxic Medicinal Plants and their ŚOdhana Process|| |
Many species of the genus Aconitum viz., Aconitum ferox Wall., Aconitum napellus Linn., and Aconitum chasmanthum Holmes ex. Stapf. are known under the common name "Vatsanābha" in Sanskrit and "Aconite" in English. The roots of all the three plants are extremely poisonous but useful in the treatment of various diseases such as fever, rheumatoid arthritis, sciatica, hypertension, and acts as "rasāyana" (immunomodulators) after their detoxification. ,, Most of the alkaloids present in the root of Aconitum species at higher doses are reported to have cardiotoxic and neurotoxic effects. Severe Aconite poisoning results mainly due to the accidental ingestion of wild plant or excess consumption of herbal decoction made from the Aconite roots. , Isolated compound (Aconite) from Vatsanābha at a dose of 2 mg can cause death, while 1 g of Vatsanābha is fatal for human being.  The root of Vatsanābha was used as poison for hunting animals in ancient times by tribals.  Overdosing of traditional Ayurvedic formulations of Vatsanābha may cause hypotension, bradycardia or bidirectional tachycardia. ,, Due to such reasons, the therapeutic dose of Vatsanābha mentioned in Ayurvedic system of medicine is 8 mg to 16 mg/day.  Its purification process includes svedana (boiling) in dola yantra using Godugdha for 3 h daily for three continuous days, followed by washing with water thrice and drying under sun light. , After Śodhana process, the total alkaloid content decreases,  but the contents of less toxic substances such as aconine, hypoaconine, and benzylhypoaconine increases , possibly due to conversion of the toxic aconitine into aconine or hydrolysis of the alkaloids to their respective amino alcohols after Śodhana process. , In another study, it has been reported that the purified form of A. carmichaeli produces cholinergic stimulation which prevents the cold-stress-induced hypothermia and immuno-suppression.  Moreover, the unpurified root of A. napellus has been reported to cause a significant rise in heart rate and changes in electrocardiogram as compared to purified Aconite. It has been reported that Gomūtra converts Aconite to a compound with cardiac stimulant property, whereas, raw Aconite showed cardiac depressant properties. ,,, Śodhana by both Gomūtra and Godugdha makes Aconite devoid of cardiac and neuro-muscular toxic effects without affecting its antipyretic activity.  A. chasmanthum is another species which is well known for its cardiac and neuro-toxicity. According to Sarkar et al. A. chasmanthum showed toxic effects, which leads to the impairment in kidney and liver functions. Śodhana with Gomūtra reduces the toxic effects of Aconite significantly. ,
In vivo and in vitro studies on frog heart showed that A. ferox has potential effect to depress the heart rate by its positive inotropic and negative chronotropic effects and these effects may be mediated through cholinergic stimulation or by direct action on the heart muscle. 
Guñjā (Abrus precatorius Linn., Family: Fabaceae) roots, seeds, and leaves have been used traditionally for their purgative, emetic, tonic, aphrodisiac, and hair growth promoting properties after being processed through Śodhana., Since ancient times, it has been used as fish poison, arrow poison and also for criminal purposes of poisoning both humans and cattle.  Abrus seeds contain a toxic lectin, abrin (an albumotoxin), a fat-splitting enzyme, a glucoside (abrussic acid), urease, abarnin, trigonelline, choline, hypaphorine, and steroidal oil that have abortive effects. ,, Abrin has a fatal dose of 0.1-1 μg/kg in humans and it is reported that boiling renders the seed harmless. , In Śodhana of Guñjā seeds, they are subjected to the svedana in dolā yantra with Godugdha or Kāñji for 3-6 h. The Śodhita material is then subjected to washing with hot water and drying under shade.  During the Śodhana process, color of the media changes due to the removal of colored materials from the endosperm of the seeds and subsequently there is loss in weight.  According to Singh et al. High performance liquid chromatography (HPLC) study of the Guñjā extract before and after the Śodhana process showed that the level of toxic hypaphorine decreases, whereas the less toxic alkaloid abrine increases. Perhaps during Śodhana process, a major part of hypaphorine might have undergone transformation into abrine by reduction of its tertiary amino group into the primary amino group. Percentage of protein present in Guñjā also reduces after Śodhana. In another study, chromatographic evaluation confirms the absence of the steroidal oil in Śodhita Guñjā seed, which is responsible for the abortifacient effect. The LD 50 dose of Guñjā was reported to increase from 2 to 5 g/kg (aśodhita) to ≥5 g/kg (Śodhita). The efficacy studies on hair growth and antibacterial effect of the Śodhita Guñjā show significant result. ,
Kupīlu (Strychnos nux-vomica Linn., Family: Loganiaceae) is extensively used in various conditions such as nervous debility, paralysis, and weakness of limbs, sexual weakness, dyspepsia, dysentery, and rheumatism after proper Śodhana., It is used as a potent rasāyana drug for old age problems.  Kupīlu has been reported to contain active alkaloids (strychnine and brucine), which are highly poisonous. , Different techniques ,,,, have been used for the analysis and quantification of strychnine and brucine in its raw and processed seeds. Kupīlu is used not only in Ayurveda but also in Chinese and Unani system of medicine after processing. ,, There are several specific Śodhana procedures, which have been adopted to purify the toxic materials from the seeds of Kupīlu., Classical method of purification includes soaking of Kupīlu seeds in liquid media (one after another) for 3-20 days. The liquid media include kāñji (soaking for 3 days), Godugdha (boiling for 3 h), Gomūtra (7 days soaking) and Goghṛta (fried till brownish red in color and swollen)  whereas traditional practitioners use castor oil (Eraṇḍa taila) instead of grita to fry  or immerse the seeds in the exudates scraped from the fresh leaves and stems of Aloe vera (ghṛtakumārī) for 15 days, followed by ginger juice (Ārdraka svarasa) for 7 days  for purification. After Śodhana process, the seeds are washed with lukewarm water where the outer seed coat and embryo are removed from the cotyledons.  Similarly in Chinese system of medicine, nux-vomica is fried with sesame oil for detoxification. , Kupīlu after Śodhana exhibits low percentage of total alkaloid content (strychnine and brucine); and the toxic loganin glycoside is eliminated. ,,, Detoxification of Kupīlu might be due to the chemical changes that causes the enhance N-oxidation and conversion of strychnine and brucine into less toxic derivatives such as isostrychnine, isobrucine, strychnine N-oxide, brucine N-oxide, and reduced level of loganic acid content of the seeds. ,,,,, The preliminary phytochemical investigation also shows significant changes in the level of phytoconstituents in different methods of Śodhana. Being acidic in nature, kāñji is a better extraction medium because it may facilitate the extraction of alkaloids and other phytochemicals. Ādraka svarasa also produces better results in reducing the toxic constituents (alkaloids) present in the seeds.  Though larger doses of strychnine are known to be lethal,  in lower doses it is known to be a stimulator.  Gomūtra Śodhita Kupīlu shows better pharmacological potency than the raw seeds. , It has also been reported that Śodhana processes of Kupīlu enhances its hepatoprotective potency. 
The detoxification study of S. nux-vomica seeds was performed by Katiyar et al. by traditional methods using aloe and ginger juice, by frying in cow ghee and by boiling in cow milk. All the treated samples were extracted with ethanol. Ethanol extracts were used for the evaluation of spontaneous motor acting (SMA), pentobarbitone-induced hypnosis, pentylenetetrazole (PTZ)-induced convoulsions, diazepam-assisted protection and morphine induced catalepsy. Ethanolic extracts of all the samples reduced SMA and inhibiting catalepsy, but seeds processed in milk showed the lowest content of strychnine, exhibited marked inhibition of PTZ induced convulsion and maximum potentiation of hypnosis. Seeds of nux-vomica were also processed in castor oil by Mitra et al. where the strychnine and brucine contents in processed and unprocessed seed of nux-vomica were estimated by HPLC. Strychnine and brucine content in the processed seed reduced up to 67.40% and 46.58% respectively as compared to unprocessed seeds. In another experiment, Mitra et al. also performed the detoxification study of nux-vomica seeds by using cow urine, cow milk and both. After the treatment, strychnine and brucine contents were determined by HPLC. Maximum reduction in the alkaloids content was found when seeds were purified in cow urine (soaking for 7 days), followed by boiling in cow milk for 3 h.
Dhattūra (Datura metel Linn., Family: Solanaceae) seeds are highly toxic and may be fatal, due to the presence of alkaloids in them. ,,, Most of the side-effects (dryness of the mouth, excessive thirst, cramps, unconsciousness, and giddiness) are due to anticholinergic property of the alkaloids present in this plant. In the purification process of Dhattūra, seeds are soaked in freshly collected Gomūtra and kept aside for 12 h. After washing, the seeds are transferred to the dolā yantra for svedana process for 3 h. The seeds are again washed with lukewarm water, allowed to dry and the seeds testa are removed. , Reduction in total alkaloid content and increase in total protein content of seed were observed after Śodhana. Complete removal of scopolamine and partial removal of hyosciamine reflects the importance of Śodhana of Dhattūra by means of which the toxic effects are removed. 
The fruit of Bhallātaka (Semecarpus anacardium Linn., Family: Anacardiaceae) is a potent drug for nervous debility, rheumatism, epilepsy, sciatica, asthma, and many more diseases.  Pericarp of the fruit contains tarry oil consisting of anacardic acid 90% and cardol 10%. Other isolated chemical constituents are bhilawanols (urushiols), semecarpol, and anacardol. ,, Bhilawanol and anacardic acids are the phytoconstituents responsible for the irritation, blisters, toxicity and contact dermatitis. ,, The Śodhana procedure of Bhallātaka includes soaking the fruits in Gomūtra, Godugdha and rubbing it on brick gravels. After removing the thalamus portions, the fruits are kept either in Gomūtra (for 7 days) or Godugdha (for 7 days), which are finally washed with water. The seeds are then shifted to a bag containing brick gravels (for 3 days), rubbed thoroughly and dried. , During the process of Śodhana of Bhāllataka, coconut oil is applied on the exposed body parts of the persons involved in the processing to reduce the chances of dermatitis.  Weight loss observed after Śodhana may be due to the reduction of the oil content of the fruits. It reveals that after Śodhana the polar constituents decrease from the plant materials. The increase in ash value may be due to the addition of brick powder with plant material.  Increase level of anacardol is observed in Śodhita fruits in comparison to the raw fruits. Recent studies prove the changes of Rf values of phytoconstituents in Śodhita samples of Bhallātaka as compared to raw Bhallātaka., Due to the decarboxylation of the oil, the anacardic acid gets converted into the less toxic anacardol. Decarboxylation process may start right from the point of cutting of the fruit and will be catalyzed in further processing.  It is possible that a greater percentage of oil might get reduced by soaking the fruits in the Gomūtra or Godugdha. Brick powder has adsorbent property because of which it absorbs irritant oils in the fruit. Śodhana does not affect the amount of total flavonoids and the total carbohydrate content; however, considerable decrease in total phenolic content was reported after the Śodhana process.  Antioxidant activity of S. anacardium decreases but the safety profile of the drug increases as the toxic phenolic oil is removed during Śodhana. In addition, after Śodhana, the plant showed normal anti-artharitic activity, that is, there was no effect of Śodhana on a desirable property. 
Karavīra (Nerium indicum; Family: Apocynaceae) has anti-stress, anti-inflammatory, antifungal, cardiotonic, neuroprotective, and anticancer activities.  This plant contains a mixture of toxic cardiac glycosides, the cardenolides , particularly oleandrin and neriine.  Roots of N. indicum are purified by svedana process in dolā yantra using Godugdha for 3 h. After Śodhana, the roots are washed with water and dried.  There is a decrease in the cardenolide and oleandrin content of the Śodhita Karavīra. Hence, the removal of these compounds may be responsible for the reduction in their effects. It was also observed that Śodhita dravya showed no reported toxicity in animal models. 
Guggula (Commiphora mukul Hook. Ex. Stocks. Engl., Family: Burseraceae) is an oleo-gum resin containing dust, dry leaves, and other foreign materials. It is recommended that it should be used after purification, which makes it safer and more effective for use. , Its purification process involves svedana in dolā yantra by using various media such as distilled water, Triphalā kvātha, Godugdha and Gomūtra. When all the Guggula dissolves in media, poṭṭalī is to be removed and the liquid is evaporated to collect Śodhita Guggula. It is indicated in the literature that Śodhana of guggula may enhance specific action such as increasing mobile property, body tonic property, and bioavailability. , Śodhita Guggula shows considerable antispasmodic activity against spasms induced by acetylcholine, histamine and barium chloride on ileum of guinea pig and Wistar rats, which are absent in aśodhita guggula. The study demonstrates that purification processes significantly modified the anti-inflammatory activities of Guggula. On the basis of toxicity study, it is concluded that after Śodhana process the toxicity of purified Guggula reduces as compare to crude Guggula.
The rhizome of Vacā 0(Acorus calamus Linn., Family: Araceae) finds use as brain tonic, appetizer, emetic, and antiepileptic. , It also possesses tranquilizing, antimicrobial, antidiarrheal, antidyslipidemic, neuroprotective, antioxidant, anticholinesterase, spasmolytic, antiulcer, anthelmintic, anti-inflammatory, and analgesic activities. ,,, Though Vacā does not come under poisonous drug category, yet some Ayurvedic texts and Ayurvedic Pharmacopoeia of India have recommended Śodhana process for Vacā rhizome. ,, The reason behind the Śodhana of Vacā though not clearly mentioned in any of the texts, may be surmised to be to reduce the tīkṣṇa, emetic and strong carminative actions of rhizome. The major active principles present in the A. calamus oil are α and β-asarone, calamene, calamenenol, calameone, α-pinene, camphene, and eugenol. , Most of the pharmacological actions of A. calamus are attributed to aromatic oils and β-asarone,  but it is carcinogenic in rodents and potentially genotoxic. The content of β-asarone in A. calamus depends on the ploidy level of the plant. The diploid variety found in North America is free from β-asarone. The triploid variety found in Europe contains 9-13% of β-asarone. The tetraploid variety of A. calamus found in India contains around 75% of β-asarone.  However, it may not be ruled out that ancient Indian physicians were well aware of its toxic effects such as carcinogenicity and mutagenecity and to overcome these effects they might have recommended the Śodhana procedure. , The Śodhana procedure involves boiling of Vacā successively by Gomūtra, Muṇḍīkvātha (decoction prepared from whole plant of Sphaeranthus indicus) and Pañcapallava kvātha for 3 h. After that it is treated with Gandhodaka for 1 h.  After Śodhana process, the rhizomes are shade dried for 12 days. Multiple processes of heating with different media lead to the decrease in the content of β-asarone due to its volatilization.  As per the study conducted by Bhat et al., pretreatment of rats with both raw and Śodhita Vacā exhibit significant anticonvulsant activity by decreasing the duration of tonic extensor phase. Further, Śodhita Vacā significantly decreases the duration of convulsion and stupor phases of MES-induced seizures. Raw Vacā shows 31.76% protection, while Śodhita Vacā show 36.48% protection at 5% level of significance. Both the samples of Vacā shorten other phases of MES-induced seizures, such as flexion, convulsion, and stupor, however, only the observed decrease of clonus and stupor in Śodhita Vacā treated group was found to be statistically significant. Subjecting to classical Śodhana procedure did not reduce the efficacy of Vacā rhizomes, instead it enhanced the activity profile of the Vacā. Toxicity studies indicate that oral administration of rhizomes of both Śodhita and raw Vacā powder at 2000 mg/kg in albino rats is relatively safe. 
Gloriosa superba Linn. (Family: Liliaceae) is a semi-woody herbaceous climber which is used in inflammations, gout, rheumatoid arthritis, gonorrhea, fever and in promoting labor pains.  The colchicine present in this plant is reported for its toxic effects, particular cardiotoxicity. ,,,, The species also contains another toxic alkaloid, gloriosine. , The Śodhana/detoxification process involve the soaking of roots and seeds in Gomūtra for 24 h and then washing with warm water.  After the Śodhana process the level of colchicine significantly reduces as colchicine is polar in nature and therefore soluble in Gomūtra and water. 
Citraka (Plumbago zeylanica Linn., Family: Plumbagi-naceae) is commonly used as appetizer, digestive, in irritable bowel disease, pain and piles.  Plumbagin at higher doses has been reported to be highly cytotoxic.  Citraka in higher dose may causes paralysis due to presence of plumbagin, and the minimum lethal dose in frog, mice and rabbits were reported to be 0.5, 0.1 and 10 mg/kg respectively. For purification process, Citraka is soaked in lime mixed with water for 24 h. The same procedure is repeated for another 24 h.  It has been reported that Śodhana of Citraka, removed 50% of plumbagin.  In another comparative study it has been reported that after the Śodhana, plumbagin content is comparatively reduced in the roots of P. zeylanica significantly as compared to roots of P. indica.
Kumbhinī (Croton tiglium) is widely used for constipation, dyspepsia, dysenteria, intestinal inflammation, and other gastrointestinal disorders.  Seeds contain an irritating oil, a toxic protein constituent, "crotin" (composed of a "crotonglobulin" and a "crotonalbumin")  and also strong purgative principles such as phorbol esters and crotonic acid. , Kumbhinī seeds are purified by svedana with Godugdha in a dolā yantra for 3 h, after removing its raphae which are later triturated with lemon juice.  The phorbol content and toxicity of the croton oil has been reported to significantly reduced, whereas its pharmacological potency increased after the Śodhana process.  Significant changes were observed in the physicochemical parameters of seeds after Śodhana. The quantity of major purgative principles phorbol ester and crotonic acid in unpurified and purified samples were determined by HPLC. The content of the phorbol ester in unpurified and purified sample was found to be 5.2 mg/100 g and 1.8 mg/100 g of dried seeds of C. tiglium, respectively. The quantity of crotonic acid in unpurified seeds of C. tiglium was found to be 0.102 mg/100 g of dried seeds. Crotonic acid content was found to be absent in the purified seed extract of C. tiglium.
The opium obtained from the fruits of Papaver somniferum Linn. is bitter, astringent, sweet, constipating, aphrodisiac, sedative, somniferous, narcotic, myotic, and antispasmodic. It is used for the treatment of cough, fever, inflammatory affections of eye, proctalgia and low back pain due to diarrhea and dysentery, migraine, malaria, dysmenorrhea, cystitis, menorrhagia, and other painful conditions.  Major constituents of opium are morphine and papavarine. Large dose of opium exhibited toxic effects of central nervous system, induces sleep, relieves pain and develops euphoria. Toxic effects of opium can be reduced by steeping in cold water for 5-6 h. After this process, the insoluble brown latex obtained is used in the Ayurvedic medicine.  Severe toxicity of opium can also be reduced by triturating with ginger juice. This process is repeated 21 times. ,,
Leaves of Cannabis seativa Linn. are bitter, astringent, tonic, aphrodisiac, alterative, intoxicating, stomachic, analgesic, and abortifacient. It is used for the treatment of convulsions, otalgia, abdominal disorders, malarial fever, dysentery, diarrhea, skin diseases, hysteria, insomnia, gonorrhea, colic, tetanus, and hydrophobia. Its excessive use causes dyspepsia, cough, impotence, melancholy, dropsy, restlessness, and insanity.  In order to reduce these toxic effects, Bhangā is boiled with Babbula Tvak kvātha for 3 h and the powder obtained is triturated with Godugdha., Toxic effects of Bhangā can also be reduced by triturating with Babbula Tvak kvātha and frying the powder obtained in Cow Ghee. 
| Conclusion|| |
As per the concept of Ayurveda, "even a strong poison can be converted to an excellent medicine if processed and administrated properly. On the other hand, even the most useful medicine may become a poison if handled incorrectly." Over time Ayurvedic practioners have tried to develop a number of traditional methods to convert toxic medicinal plants to useful medicines. Ancient healers, who developed formulations based on medicinal herbs, were probably not aware of the chemical composition of the herbs. Ayurveda uses many toxic natural drugs either in their crude form or after subjecting them to Śodhana processes for treating human ailments. It may be concluded that the traditional system of purification (Śodhana) can influence the phytochemical, pharmacological, and toxicological profile of the plant drugs and thereby useful in increasing safety profile and efficacy of the drugs. It is worthwhile to adopt Śodhana processes as per Indian system of medicine in the development of herbal formulations with application of modern technology to assess its safety and efficacy. Studies have shown that the toxic constituents are transferred into media rendering the drug nontoxic. Specific media has definitely an important role in making a drug act without causing side-effects/adverse effects.
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| References|| |
Saklani A, Kutty SK. Plant-derived compounds in clinical trials. Drug Discov Today 2008;13:161-71.
Butler MS. The role of natural product chemistry in drug discovery. J Nat Prod 2004;67:2141-53.
Ninan B, Wertheimer AI. Withdrawing drugs in the US versus other countries. Inov Pharm 2012;3:1-12.
Sharma PV. Dravyaguna Vijnana. Golden Jubilee Edition. Varanasi: Chaukhambha Surabharati Academy; 2008. p. 128.
Ministry of Health and Family Welfare (Department of Health). Drugs and Cosmetics act 1940 with Drugs and Cosmetics Rules, 1945. New Delhi: Ministry of Health and Family Welfare (Department of Health), Government of India; 2003. p. 317.
Mishra GS. Àyurveda Prakash. New Delhi, India: Chaukhamba Surabharati Academy; 2007. p. 490-5.
Acharya JT. Agnivesa: Caraka Samhita. Varanasi: Chawkhambha Vidyabhawan; 2011. p. 23.
Belge RS, Belge AR. Ayurvedic Shodhanna treatments and their applied aspect with special reference to Loha. J Pharm Biol Sci 2012;2:45-9.
Mitra S, Shukla VJ, Acharya R. Effect of Shodhana (processing) on Kupeelu (Strychnos nux-vomica
Linn.) with special reference to strychnine and brucine content. Ayu 2011;32:402-7.
Patel Y, Bhat SD, Rabinarayan A, Ashok BK, Shukla VJ. Role of Shodhana on analytical parameters of Datura innoxia
Mill and Datura metel
Linn. seeds. Int J Res Ayurveda Pharm 2010;1:249-54.
Sarkar PK, Prajapati PK, Shukla VJ, Ravishanka B. Evaluation of effect of Shodhana process on pharmacological activities of aconite. Indian J Pharm Educ Res 2012;46:243-7.
Mishra BS. Yogaratnakara. Varanasi: Choukambha Prakashana; 2010. p. 167-9.
Chaube A, Prajapati PK, Dixit SK. On the technique of sodhana. Anc Sci Life 1996;16:67-73.
Chaudhary A, Singh N. Herbo mineral formulations (rasaoushadhies) of ayurveda an amazing inheritance of ayurvedic pharmaceutics. Anc Sci Life 2010;30:18-26.
Kamble R, Sathaye S, Shah DP. Evaluation of antispasmodic activity of different shodhit guggul using different shodhan process. Indian J Pharm Sci 2008;70:368-72.
Ilanchezhian R, Roshy JC, Acharya R. Importance of media in shodhana (purification/processing) of poisonous herbal drugs. Anc Sci Life 2010;30:54-7.
Ffrench G. Aconitine-induced cardiac arrhythmia. Br Heart J 1958;20:140-2.
Makino T, Kato K, Mizukami H. Processed aconite root prevents cold-stress-induced hypothermia and immuno-suppression in mice. Biol Pharm Bull 2009;32:1741-8.
Rastogi S. A review of aconite (Vatsanabha
) usage in Ayurvedic formulations: Traditional views and their references. Spatula DD 2011;1:233-44.
Singh S, Fadnis PP, Sharma BK. Aconite poisoning. J Assoc Physicians India 1986;34:825-6.
Chan TY. Aconite poisoning. Clin Toxicol (Phila) 2009;47:279-85.
Panda AK, Debnath SK. Overdose effect of aconite containing Ayurvedic Medicine (′Mahashankha Vati′). Int J Ayurveda Res 2010;1:183-6.
Shyaula SL. Phytochemicals, traditional uses and processing of Aconitum
species in Nepal. Nepal J Sci Technol 2011;12:171-78.
Tai YT, Lau CP, But PP, Fong PC, Li JP. Bidirectional tachycardia induced by herbal aconite poisoning. Pacing Clin Electrophysiol 1992;15:831-9.
Rastogi S, Ranjana SR. Adverse effects of Ayurvedic drugs: An overview of causes and possibilities in reference to a case of Vatsanabha
0 (Aconite) overdosing. Int J Risk Saf Med 2007;19:117-25.
Sastri A. Vagbhattacharya: Rasaratna Samuchchaya. 6 th
ed. Varanasi: Chaukhamba Sanskrit Series Office; 1978. p. 590.
Sarkar PK, Prajapati PK. Dispense with Ayurvedic samskara: Shodhana of aconite. Indian Drugs 2011;48:31-44.
Shastri K. Sadananda Sharma: Rasa Tarangini. 11 th
ed. New Delhi: Motilal Banarasidas; 2012. p. 651-52.
Singh LB. Poisonous (Visa) Plants in Ayurveda. 2 nd
ed. Varansai: Chaukhamba Sanskrit Bhawan; 2003.
Deore SL, Moon KV, Khadabadi SS, Deokate UA, Baviskar BA. Evaluation of toxicity of ′Vatsanabha′
) Before and After Shodhana. J Young Pharm 2013;5:3-6.
Handa KL, Chopra IC, Kohli JD, Singh K. Mitigation of aconite; a preliminary note. Indian J Med Res 1951;39:89-98.
Parikh KM, Doshi VJ, Salunkhe UB, Dhanvate AA. Authentication of detoxification process used in traditional Indian medicine. Int Hortic Soc Acta Hortic 1996;426:57-4.
Paul A. Effects of Avurvedic shodhana (processing) on dried tuberous Aconite
0 (Aconitum napellus
Linn.) root. Indones J Pharm 2013;24:40-6.
Tai YT, But PP, Young K, Lau CP. Cardiotoxicity after accidental herb-induced aconite poisoning. Lancet 1992;340:1254-6.
Sarkar PK, Prajapati PK, Shukla VJ, Ravishankar B. Effect of shodhana treatment on chronic toxicity and recovery of aconite. Toxicol Int 2012;19:35-41.
Sahoo S, Swain TR, Dash NC. Study on the pharmacological profile of purified Aconitum ferox
extracts in Frog. Int J Res Pharm Biomed Sci 2013;4:746-53.
Sreeramulu J, Reddy JR, Reddy YP, Geethavani M. Antimicrobial activity of seeds of Abrus precatorius
Linn. Asian J Chem 2009;21:1630-2.
Acharya R, Roy S. A Review on therapeutic utilities and purificatory procedure of gunja (Abrus precatorius
Linn.) as described in Ayurveda.
J Agric Sci Technol 2013;2:1-11.
Olsnes S. The history of ricin, abrin and related toxins. Toxicon 2004;44:361-70.
Chauhan NS. Medicinal and Aromatic Plants of Himachal Pradesh. New Delhi: Indus Publication Company; 1999. p. 49-52.
Dimetry NZ, Gengaihi SE, Reda AS, Amer SA. Biological effect of some isolated Abrus precatorius
L. alkaloids towards Tetranychus urticae
Koch. Anz Schadlingskunde Pflanzenschutz Umweltscgutz 1992;65:99-101.
Ross IA. Medicinal Plants of the World. Chemical Constituents, Traditional and Modern Medicinal Uses. 2 nd
ed., Vol. 1. Totowa, NJ: Humana Press Inc.; 2003. p. 15-31.
Parikh CK. Parikh′s Test Book of Medical Jurisprudence Forensic Medicine and Toxicology. 6 th
ed. Darya Ganj (New Delhi): CBS Publishers and Distributors (India); 2007. p. 9.31-11.16.
Kekuda TR, Vinayaka KS, Soumya KV, Ashwini SK, Kiran R. Antibacterial and antifungal activity of methanolic extract of Abrus pulchellus
Wall. and Abrus precatorius
Linn.: A comparative study. Int J Toxicol Pharmacol Res 2010;2:26-9.
Roy S, Acharya R, Mandal NC, Barman S, Ghosh R, Roy R. A comparative antibacterial evaluation of raw and processed Guñja (Abrus precatorius
Linn.) seeds. Anc Sci Life 2012;32:20-3.
Singh GD, Banerji R, Mahrotra S. Effect of shodhana on the toxicity of Abrus precatorius
. Anc Sci Life 1998;18:127-9.
Barve KH, Ojha N. Effective detoxification of Abrus precatorius
Linn. seeds by Shodhana. J Ayurveda Integr Med 2013;4:82-5.
Lavekar GS, Padhi MM, Joseph GV, Selvarajan S, Mangal AK, Sharma PC, et al
. Database on Medicinal Plants used in Ayurveda. Vol. 5, 9. New Delhi, India: Central Council for Research in Ayurveda and Siddha; 2008. p. 139-63.
Mitra S, Kumar V, Ashok B, Acharya RN, Ravishankar B. A comparative anti-inflammatory activity of raw and processed Kupeelu (Strychnos nux-vomica
Linn.) seeds on albino rats. Anc Sci Life 2011;31:73-5.
Pandey G. Anti-aging Herbal Drugs of India. 1 st
ed. Delhi: Sri Satguru Publication; 2002. p. 248.
Sarvesvaran R. Strychnine poisoning: A case report. Malays J Pathol 1992;14:35-9.
Ko RJ. A U.S. perspective on the adverse reactions from traditional Chinese medicines. J Chin Med Assoc 2004;67:109-16.
Kamal A, Kamal YT, Ahmad S, Ahmad FJ, Saleem K. Simultaneous HPTLC determination of strychnine and brucine in Strychnos nux-vomica
seed. J Pharm Bioallied Sci 2012;4:134-9.
Choi YH, Sohn YM, Kim CY, Oh KY, Kim J. Analysis of strychnine from detoxified Strychnos nux-vomica
[corrected] seeds using liquid chromatography-electrospray mass spectrometry. J Ethnopharmacol 2004;93:109-12.
Kaye S, Hoff EC. Identification and determination of strychnine by Ultra‒violet spectrophotometry. J Crim Law Criminol Police Sci 1952;43:246-9.
Duverneuil C, de la Grandmaison GL, de Mazancourt P, Alvarez JC. Liquid chromatography/photodiode array detection for determination of strychnine in blood: A fatal case report. Forensic Sci Int 2004;141:17-21.
Li Y, He X, Qi S, Gao W, Chen X, Hu Z. Separation and determination of strychnine and brucine in Strychnos nux-vomica
L. and its preparation by nonaqueous capillary electrophoresis. J Pharm Biomed Anal 2006;41:400-7.
Cai BC, Yang WX, Zhu WY, Lu JC, Ye DJ. Effect of processing on the extraction of alkaloids from Strychnos
. Zhongguo Zhong Yao Za Zhi 1993;18:23-4, 62.
Akbar S, Khan SA, Masood A, Iqbal M. Use of Strychnos nux-vomica
0 (Azraqi) seeds in Unani system of medicine: Role of detoxification. Afr J Tradit Complement Altern Med 2010;7:286-90.
Mehta N, Prajapati PK, Chadhuary AK. Role of milk in Shodhana (detoxifcation) with special reference to nux-vomica. Aryavaidyan 2007;20:100-4.
Yadavji TA. Siddhayog Samgraha. 13 th
ed. Allahabad: Shree Baidyanath Ayurved Bhavan Ltd.; 2008. p. 163.
Katiyar C, Kumar A, Bhattacharya SK, Singh RS. Ayurvedic processed seeds of nux-vomica: Neuropharmacological and chemical evaluation. Fitoterapia 2010;81:190-5.
Mitra S, Acharya R, Shulka VJ. Role of castor oil in processing (Shodhana) of kupeelu (Strychnos nux vomica Linn.
) Seeds: An approach of traditional Ayurveda. Int J Ayurvedic Med 2011;2:62-71.
Gopalkrishna SV, Lakshmi NM, Ramachandra SS. Hepatoprotective activity of detoxified seeds of nux-vomica against CCl4 induced hepatic injury in albino rats. Pharmacologyonline 2010;1:803-15.
Chung B, Shin MK. Dictionary of folk medicine. Seoul: Young Lim Press; 1989. p. 972.
Joshi D, Agrawal K. Effect of purification (Shodhana) on the alkaloidal concentration of kuchala seeds. J Res Indian Med Yoga Homeopath 1977;12:43-6.
Kumar A, Sinha BN. Ayurvedic processings of nux‒vomica: Qualitative and quantitative determination of total alkaloidal contents and relative toxicity. Malays J Pharm Sci 2009;7:83-98.
Cai BC, Hattori M, Namba T. Processing of nux vomica. II. Changes in alkaloid composition of the seeds of Strychnos nux-vomica
on traditional drug-processing. Chem Pharm Bull (Tokyo) 1990;38:1295-8.
Wu W, Qiao C, Liang Z, Xu H, Zhao Z, Cai Z. Alkaloid profiling in crude and processed Strychnos nux-vomica
seeds by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. J Pharm Biomed Anal 2007;45:430-6.
Han QB, Li SL, Qiao CF, Song JZ, Cai ZW, Pui-Hay But P, et al.
A simple method to identify the unprocessed Strychnos
seeds used in herbal medicinal products. Planta Med 2008;74:458-63.
Jakson TA, Marsh FP. Test Methods for Vertebrate Pest Control and Management Material. American Society for Testing and Materials (Publishers); 1997. p. 1-4.
Samulesson G. Drugs of Natural Origin. Stockholm: Swedish Pharmaceutical Press; 1992. p. 282.
Kumar A, Sinha BN, Raghubir R, Nath C. Antinociceptive activity of processed and unprocessed seeds of Strychnos nux-vomica
. Ann Conf Indian Pharmacol Soc 2006;39:46.
Kumar A, Sinha BN, Raghubir R, Puri A, Srivastava A, Choudhary A. Ayurvedic processing′s of nux-vomica: Studies on appetite and antihyperlipidemic properties of processed and unprocessed seeds of Strychnos nux-vomica
in the high fat diet treated hamster model. Int Semin Ayurveda 2006;5:29.
Mitra S, Shukla VJ, Acharya R. Impact of (purificatory procedures) on kupeelu (Strychnos nux
) seeds: A pharmaceutico-analytical study. J Res Educ Indian Med 2012;18:65-71.
Boumba VA, Mitselou A, Vougiouklakis T. Fatal poisoning from ingestion of Datura stramonium
seeds. Vet Hum Toxicol 2004;46:81-2.
Pereira CA, Nishioka Sde D. Poisoning by the use of Datura
leaves in a homemade toothpaste. J Toxicol Clin Toxicol 1994;32:329-31.
Chang SS, Wu ML, Deng JF, Lee CC, Chin TF, Liao SJ. Poisoning by Datura
leaves used as edible wild vegetables. Vet Hum Toxicol 1999;41:242-5.
Kovatsis A, Flaskos J, Nikolaidis J, Kotsaki-Kovatsi VP, Papaioannou N, Tsafaris F. Toxicity study of the main alkaloids of Datura
species in broilers. Food Chem Toxicol 1993;31 Suppl 11:841-5.
Raut AK, Sawant NS, Badre AS, Amonkar AJ, Vaidya AD. Bhallatak (Semecarpus anacardium
Linn.) - A review. Indian J Tradit Knowl 2007;6:653-9.
Gedam PH, Sampathkumaran PS, Sivasamban MA. Composition of bhilawanol from Semecarpus anacardium
. Indian J Phytochem 1974;13:513-5.
Nagabhushana KS, Umamaheshwari S, Tocoli FE, Prabhu SK, Green IR, Ramadoss CS. Inhibition of soybean and potato lipoxygenases by bhilawanols from bhilawan (Semecarpus anacardium
) nut shell liquid and some synthetic salicylic acid analogues. J Enzyme Inhib Med Chem 2002;17:255-9.
Khare CP. Indian Medicinal Plants, an Illustrated Dictionary. 1 st
ed. New Delhi: Springer (India) Private Limited; 2007. p. 597.
Goldsmith NR. Dermatitis from Semecarpus anacardium
(Bhilawanol or the marking nut) spread by contaminated mail. J Am Med Assoc 1943;123:27.
Shankar DS. Contact urticaria induced by Semecarpus anacardium
. Contact Dermatitis 1992;26:200.
Llanchezhian R, Joseph C R, Rabinarayan A. Urushiol-induced contact dermatitis caused during Shodhana (purificatory measures) of Bhallataka (Semecarpus anacardium
Linn.) fruit. Ayu 2012;33:270-3.
Chunekar KC, Pandey GS. Bhavaprakasha Nighantu. 10 th
ed. Varanasi: Chaukhamba Surabharati Academy; 2006. p. 329-31.
Ilanchezhian R, Acharya RN, Roshy JC, Shukla VJ. Impact of Ayurvedic Shodhana (purificatory procedures) on Bhallataka fruits (Semecarpus anacardium
Linn.) By measuring the anacardol content. Glob J Res Med Plants Indig Med 2012;1:286-94.
Venkateshwarlu G, Saraswathi P, Shantha TR, Kishore KR, Sridhar BN. A preliminary study on the effect of traditional Ayurvedic purifying methods of Semecarpus anacardium
Linn. Nuts - A Physico, chemical and powder microscopic study. J Herb Med Toxicol 2010;4:237-47.
Deborah SG, De Oliveira ME. Effect of cashew nut processing on the burning sensation caused by anacardic acids. Proc Interam Soc Trop Hortic 2003;47:141-2.
Gajjar U, Khambholja K, Patel R. Effect of shodhana process on quantity of phytoconstituents of Semecarpus anacardium
Linn. Int J Pharm Life Sci 2011;2:805-7.
Gajjar U, Khambholja KM, Patel RK, Pancholi SS. Improvement in safety profile of Semecarpus anacardium
Linn by Shodhana - An Ayurvedic purification method. Planta Med 2013;79:P64.
Prakash D, Bindal MC, Gupta SK, Gupta AK, Vedpal. Antiarthritic activity of milk extract of Semecarpus anacardium
nut. Int Res J Pharm 2013;4:158-60.
Daware P. A pharmacological screening of Nerium oleander
Linn: A review. Int J Pharm Sci Rev Res 2012;15:125-6.
Langford SD, Boor PJ. Oleander toxicity: An examination of human and animal toxic exposures. Toxicology 1996;109:1-13.
Khan I, Kant C, Sanwaria A, Meena L. Acute cardiac toxicity of nerium oleander/indicum poisoning (kaner) poisoning. Heart Views 2010;11:115-6.
Galey FD, Holstege DM, Plumlee KH, Tor E, Johnson B, Anderson ML, et al.
Diagnosis of oleander poisoning in livestock. J Vet Diagn Invest 1996;8:358-64.
Banerjee AA, Vasu KK, Pancholi H, Rajani M, Nivsarkar MA. Detoxification of Nerium indicum
roots based on Indian system of medicine: Phytochemical and toxicity evaluations. Acta Pol Pharm 2011;68:905-11.
Anonymous. Ayurvedic Pharmacopoeia of India. Vol. 1. New Delhi: Ministry of Health and Family Welfare, Department of Indian System of Medicine and Homeopathy, Govt. of India; 1999. p. 43.
Mukherjee PK, Verpoorte R. GMP for Botanicals. 1 st
ed. New Delhi: Business Horizons, Pharmaceutical Publishers; 2003. p. 158-61.
Rajpal V. Standardization of Botanicals. New Delhi: Eastern Publishers; 2002. p. 83-91.
Karan M, Sarup P, Suneja V, Vasisht K. Effect of traditional Ayurvedic purification processes (sodhanvidhi) of guggulu on carrageenan-induced paw oedema in rats. J Pharm Biomed Sci 2012;21:1-5.
Taru P, Abhyankar M, Undale V, Bhosale A. Acute and subacute toxicity studies on Shodhana
processed guggul. Int J Pharm Sci Res 2013;4:796-9.
Anonymous. The Ayurvedic Pharmacopoeia of India. Part-1. Vol. 2. New Delhi: Govt. of India, Ministry of Health and family Welfare, Department of ISM and H; 1999. p. 177-9.
Raja AE, Vijayalakshmi M, Devalarao G. Acorus calamus
Linn.: Chemistry and biology. Res J Pharm Technol 2009;2:256-61.
Shoba FG, Thomas M. Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhoea. J Ethnopharmacol 2001;76:73-6.
Shukla PK, Khanna VK, Ali MM, Maurya RR, Handa SS, Srimal RC. Protective effect of Acorus calamus
against acrylamide induced neurotoxicity. Phytother Res 2002;16:256-60.
Tripathi ID. Chakrapanidatta: Chakradatta. Varanasi: Chawkhambha Sanskrit Bhavana; 2010. p. 155.
Shastri AD. Govind Das: Bhaisja Ratanavali. Varanasi: Chaukhambha Sanskrit Samsthan; 2008. p. 155.
Raina VK, Srivastava SK, Syamasunder KV. Essential oil composition of Acorus calamus
L. from the lower region of the Himalayas. Flavour Fragr J 2003;18:18-20.
McGaw LJ, Jager AK, Van Staden J. Isolation of beta-asarone, an antibacterial and anthelmintic compound from Acorus calamus
in South Africa. S Afr J Bot 2002;68:31-35.
Lander V, Schreier P. Acorenone and gamma-asarone: Indicators of the origin of calamus oils (Acorus calamus
L.). Flavour Fragr J 1990;5:75-9.
Bhat SD, Ashok BK, Acharya RN, Ravishankar B. Anticonvulsant activity of raw and classically processed Vacha (Acorus calamus
Linn.) rhizomes. Ayu 2012;33:119-22.
Gholkar MS, Mulik MB, Laddha KS. Fate of ß-asarone in Ayurvedic Sodhana process of Vacha. J Ayurveda Integr Med 2013;4:19-22.
Bhat DS, Ashok BK, Acharya RN, Ravishankar B. A comparative acute toxicity evaluation of raw and classically processed rhizomes of Vacha (Acorus calamus
Linn.). Indian J Nat Prod Resour 2012;3:506-11.
Samanta AK, Kumar UK. Poisoning by Glory Lily ‒ A case report. J Indian Acad Forensic Med 2005;27:188-9.
Nagaratnam N, De Silva DP, De Silva N. Colchicine poisoning following ingestion of Gloriosa superba
tubers. Trop Geogr Med 1973;25:15-7.
Dunuwille R, Balasubramaniam K, Bibile SW. Toxic principles of Gloriosa superba
. Ceylon J Med Sci 1968;17:1-7.
Eddleston M, Persson H. Acute plant poisoning and antitoxin antibodies. J Toxicol Clin Toxicol 2003;41:309-15.
Mendis S. Colchicine cardiotoxicity following ingestion of Gloriosa superba
tubers. Postgrad Med J 1989;65:752-5.
Angunawela RM, Fernando HA. Acute ascending polyneuropathy and dermatitis following poisoning by tubers of Gloriosa superba
. Ceylon Med J 1971;16:233-5.
Gooneratne BW. Massive generalized alopecia after poisoning by Gloriosa superba
. Br Med J 1966;1:1023-4.
Nabar MP, Mhaske PN, Pimpalgaonkar PB, Laddha KS, Gloriosa superba
roots: Content change of colchicine during Sodhana (detoxification) process. Indian J Tradit Knowl 2013;12:277-80.
Harborne JB, Baxter H. Phytochemical Dictionary. 1 st
ed. London: Taylor and Francis; 1995.
Alam M, Dasan KKS, Rukmani B, Purushothaman KK. Chemical, microbiological and comparative fermentation studies on Dasamularishta Purushothaman. Anc Sci Life 1984;4:123-6.
Unnikrishnan KP, Raja SS, Balachandran I. A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica
and Plumbago zeylanica
. Indian J Pharm Sci 2008;70:844-7.
Stirpe F, Pession-Brizzi A, Lorenzoni E, Strocchi P, Montanaro L, Sperti S. Studies on the proteins from the seeds of Croton tiglium
and of Jatropha curcas
. Toxic properties and inhibition of protein synthesis in vitro. Biochem J 1976;156:1-6.
Hu J, Gao WY, Gao Y, Ling NS, Huang LQ, Liu CX. M3 muscarinic receptor- and Ca 2+
influx-mediated muscle contractions induced by croton oil in isolated rabbit jejunum. J Ethnopharmacol 2010;129:377-80.
Jasicka-Misiak I, Wieczorek PP, Kafarski P. Crotonic acid as a bioactive factor in carrot seeds (Daucus carota L.). Phytochemistry 2005;66:1485-91.
Khandelwal KR, Thonte SS. Effect of "Shodhana" (purification) on the toxicity of croton oil. Indian J Nat Prod 1993;9:10-1.
Kishore KR, Shantha TR, Rao VR, Venkateshwarlu G, Sridhar BN. Evaluation of Shodhana on Jayapala (Croton tiglium Linn.) seeds: A preliminary physico‒phyto‒chemical analysis. J Herb Med Toxicol 2013;7:79-87.
Pal PK, Nandi MK, Singh NK. Detoxification of Croton tiglium L. seeds by Ayurvedic process of Sodhana. Anc Sci Life 2014;33:157-61.
Manna SK, Sah NK, Newman RA, Cisneros A, Aggarwal BB. Oleandrin suppresses activation of nuclear transcription factor-kappaB, activator protein-1, and c-Jun NH2-terminal kinase. Cancer Res 2000 15;60:3838-47.
Patil S. A critical review on Upavisa with special reference to their therapeutics. J Biol Sci Opin 2014;2:196-202.
Krishna PS. Rasendra Sara Samgraha. Varanasi: Krishnadas Academy; 1994. p. 218.
Parikh GN. Vanousadhi Rathnakara. Vol. II. 2 nd
ed. Vijaygarh: Sudhanidhi Karyalaya; 1990. p. 370-2.
[Table 1], [Table 2], [Table 3]
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