Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Users Online: 65 | Home Print this page Email this page Small font size Default font size Increase font size
ORIGINAL ARTICLE
Year : 2017  |  Volume : 37  |  Issue : 2  |  Page : 74-80

Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik


Division of Pharmaceutical Chemistry, Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India

Correspondence Address:
Adikay Sreedevi
Division of Pharmaceutical Chemistry, Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/asl.ASL_173_17

Rights and Permissions

Aim: The present study was undertaken to evaluate the potential role of ethanol extract of seeds of Lens culinaris in attenuation of Cisplatin-induced nephrotoxicity in male Albino rats. Materials and Methods: Seeds of Lens culinaris were powdered, defatted and ethanol extract was prepared by hot extraction method. The ethanol extract so prepared was subjected to preliminary phytochemical evaluation and fingerprint analysis using high performance thin layer chromatography. Nephroprotective potential of extract was evaluated at 200 and 400 mg/kg b.w. in both curative and prophylactic regimens in male Albino rats. Nephrotoxicity was induced by single intra peritoneal injection of Cisplatin at a dose of 5 mg/kg b.w. Nephroprotective activity was assessed by determining serum markers, urinary parameters, lipid peroxidation and antioxidant levels in renal tissue. Results: Cisplatin had induced marked acute renal toxicity as manifested by a significant increase in serum marker levels, urinary total protein, lipid peroxidation and decrease in urinary creatinine, catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH). The administration of extract significantly attenuated the Cisplatin-induced nephrotoxicity remarkably by restoring the biochemical and oxidative stress markers in both curative and prophylactic regimens in a dose dependent manner. Histological and immunohistochemical studies also substantiated the biochemical studies. Conclusion: The findings of the present study provides corroborative scientific evidence for the attenuation of Cisplatin-induced nephrotoxicity by seeds of Lens culinaris and validates the ethno-medicinal use of this seeds as renoprotective agent.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1254    
    Printed59    
    Emailed0    
    PDF Downloaded62    
    Comments [Add]    

Recommend this journal