Ancient Science of Life

ARTICLE
Year
: 2003  |  Volume : 22  |  Issue : 4  |  Page : 134--139

In-vitro culture studies for callus and root generation of Boerhaavia diffusa Linn


Vandana Jain1, D Singh1, Swarnalata Saraf1, S Saraf2 
1 B.R. Nahata College of Pharmacy, Mandsaur-458 001, M.P., India
2 Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur-492 010. C.G., India

Correspondence Address:
Vandana Jain
B.R. Nahata College of Pharmacy, Mandsaur-458 001, M.P.
India

Leaf and stem explants excised from young plant of B.diffusa  were cultured on Murashige and Skoog (MS) medium containing agar (0.8%), sucrose (2.5%) and varied concentration of Indole butyric acid (IBA), Napthyl acetic acid (NAA), 2,4 - dichloro phenoxy acetic acid (2,4-D) and Picrolam. Leaf explants has given better response for both rooting and callus formation. IBA in a concentration of 5μM has shown maximum regeneration (69.7%) with induction period of 7 days. The developed roots were similar to that of naturally grown roots with little anatomical changes. For callus formation 20μM 2, 4-D has given maximum amount and percentage response 979.5% with an induction period of 8 days. Picrolam (10μM) has shown 36.6% response and the average weight of callus was less as compared with 2,4-D. The callus obtained was friable and opaque in nature.


How to cite this article:
Jain V, Singh D, Saraf S, Saraf S. In-vitro culture studies for callus and root generation of Boerhaavia diffusa Linn.Ancient Sci Life 2003;22:134-139


How to cite this URL:
Jain V, Singh D, Saraf S, Saraf S. In-vitro culture studies for callus and root generation of Boerhaavia diffusa Linn. Ancient Sci Life [serial online] 2003 [cited 2020 Aug 3 ];22:134-139
Available from: http://www.ancientscienceoflife.org/article.asp?issn=0257-7941;year=2003;volume=22;issue=4;spage=134;epage=139;aulast=Jain;type=0