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ARTICLE
Year : 1996  |  Volume : 16  |  Issue : 1  |  Page : 74-78

Effect of L-dopa and L- methionine supplementation on bioproduction of ementine in callus cultures of Cephaelis ipecacuanha


Periyar College of pharmaceutical sciences for Girls, Trichy- 620 021, India

Correspondence Address:
T Vetrichelvan
Periyar College of pharmaceutical sciences for Girls, Trichy- 620 021
India
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Source of Support: None, Conflict of Interest: None


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Callus cultures were induced from leaf segments of Cephaelis ipecacuanha  on modified gamborg medium containing 2,4 - dichlorophenoxy acetic acid (2mg/1) Indole acetic acid (1mg/1), kinetin (1mg/1) and sucrose (20g/1). The biogenetic profile for alkaloids was established. The established callus cultures were transferred to modified gamborg medium supplemented with L- DOPA (40 mg/1) and L methionine (3mg/1). After four weeks of their growth, the biomass and medium were extracted for emetine alkaloid and analysed by HPLC. The bioproduction of emetine was found to be extracellular in nature. The emetine content had increased to 0.587% mg FW as compared to control cultured (0.0245% mg FW).


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