Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Users Online: 830 | Home Print this page Email this page Small font size Default font size Increase font size
Year : 2013  |  Volume : 32  |  Issue : 6  |  Page : 17

OA02.11. Alpha‑amylase inhibitory property and antioxidant potential of Saraca indica and Polyalthia longifolia bark extracts

1 Tamil Nadu Agricultural University, Coimbatore, India
2 Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, India

Correspondence Address:
P Gayathri
Tamil Nadu Agricultural University, Coimbatore
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0257-7941.123831

Rights and Permissions

Purpose: The present study was designed to study the alphaamylase inhibitory property and antioxidant potential of the various solvent extracts of the bark of two medicinal plants Saraca indica and Polyalthia longifolia. Method: The yield percentage of aqueous, ethanol, ethyl acetate, hexane and chloroform extracts of Saraca indica and Polyalthia longifolia bark were assessed by extracting 5 g of the dried plant material in 25 ml of the respective solvent in a shaker at 40 degree celsius for 48 hours. Filtered and evaporated the solvents at room temperature. Aqueous extract was subjected to lyophilization. The residues were weighed to calculate the yield. Total antioxidant potential of the extracts were assessed by FRAP assay and compared with standard ascorbic acid. Inhibition of in vitro lipid peroxidation of the potent extract was studied using linolenic acid micelles as model system. Alphaamylase inhibitory property of the extracts was studied by the method given by Bernfield (1995) and compared with that of the standard inhibitor acarbose. Also the type of inhibition of the potent extracts was studied by Dixon plot. Result: The results revealed that the yield percentage was highest for ethanolic extracts of Saraca indica and Polyalthia longifolia bark. Among all the tested extracts the ethanolic extract of both the barks found to possess highest antioxidant potential and alpha amylase inhibitory activity followed by the aqueous and ethylacetate extracts. Hexane and chloroform extracts possessed lowest antioxidant and amylase inhibition potential. The ethanolic extracts presented good inhibition of in vitro lipid peroxidation. The type of inhibition of amylase showed by the ethanolic extracts was found to be competitive. Conclusion: To conclude, the ethanolic extracts of Saraca indica and Polyalthia longifolia bark were found to possess good antioxidant potential and also showed efficient inhibition of the enzyme alphaamylase.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded60    
    Comments [Add]    

Recommend this journal