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ORIGINAL ARTICLE
Year : 2014  |  Volume : 34  |  Issue : 2  |  Page : 85-88

Efficacy of garlic extract and chlorhexidine mouthwash in reduction of oral salivary microorganisms, an in vitro study


Department of Paedodontics and Preventive Dentistry, Yenepoya Dental College, Yenepoya University, Deralakatte, Mangalore, Karnataka, India

Correspondence Address:
H T Ajay Rao
Department of Paedodontics and Preventive Dentistry, Yenepoya Dental College, Yenepoya University, University Road, Nithyananda Nagar Post, Deralakatte, Mangalore - 575 018, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0257-7941.153465

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Objectives: To assess and compare the antimicrobial effect of garlic extract and chlorhexidine (CHX) mouthwash solution against oral salivary microorganisms. Materials and Methods: Thirty six salivary samples were obtained in dry plastic vials. Collected saliva samples were centrifuged. Each vial was mixed completely on a shaker after which 1 ml of saliva was added to 9 ml of ethanol by a sterile pipette and mixed. A volume of 1 ml of garlic hydro-alcoholic extract and 1 ml of mouthwash was added to 1 ml each case specimen and was transferred to culture medium of Trypticase Soy Agar. Agar plates were incubated at 37°C for 48 h to allow for microbial growth. Microbial colonies were counted by independent interpreter to evaluate the result. Statistical Analysis: Data obtained were analyzed using one-way ANOVA test. P < 0.001 was considered statistically significant. Result: Result of the study shows that mean colony count of salivary microbial population was (1984 ± 400) 1127 in saline group (negative control), (50 ± 4) 27 in (0.12%) CHX group (positive control), (700 ± 200) 469 in garlic extract (5%) group (case control). Conclusion: Mouthwash containing garlic extract can be used as an alternative to CHX mouthwash.


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